Last data update: May 13, 2024. (Total: 46773 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Ayinmode AB[original query] |
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Molecular characterization of Cryptosporidium in children in Oyo State, Nigeria: implications for infection sources
Ayinmode AB , Fagbemi BO , Xiao L . Parasitol Res 2012 110 (1) 479-81 A study was conducted to detect and identify Cryptosporidium spp. in 43 children from Oyo State, Nigeria. Using nested polymerase chain reaction, 11.6% of the children were identified as positive for Cryptosporidium spp. Restriction fragment length polymorphism analysis and DNA sequencing of the PCR products showed the presence of three subtype families of Cryptosporidium hominis (two isolates of Ia and one isolate of Ib) and Cryptosporidium parvum (two isolates of IIc), all anthroponotic in nature. This study identified a high diversity of Cryptosporidium subtypes and clearly suggested that anthroponotic rather than zoonotic transmission played a more important role in the epidemiology of Cryptosporidium in the studied area. |
Molecular identification of Enterocytozoon bieneusi isolates from Nigerian children
Ayinmode AB , Ojuromi OT , Xiao L . J Parasitol Res 2011 2011 129542 A study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three known genotypes (two isolates of genotype D and one of genotype K) and one new genotype. This study suggests that either human or animal (or both) could be the infection source for the children, since identified genotypes D and K have been previously detected in both immunocompromised and immunocompetent patients and domestic animals. The identification of high diversity also suggests intensive transmission of microsporidiosis in the studied area. |
Molecular characterization of Cryptosporidium spp. in native calves in Nigeria
Ayinmode AB , Olakunle FB , Xiao L . Parasitol Res 2010 107 (4) 1019-21 Most studies on the distribution of Cryptosporidium species in cattle were done with dairy breeds in industrialized nations. In this study, 65 fecal samples from randomly selected 12-24-week-old diarrheic calves in four white Fulani herds in southwestern Nigeria were screened for Cryptosporidium spp. using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the small subunit rRNA gene. Thirty-four (52.3%) of the samples were positive for Cryptosporidium. RFLP analysis of PCR products showed that 18 (27.7%) and five (7.7%) of the positive samples had Cryptosporidium bovis and Cryptosporidium ryanae, respectively, and 11 (16.9%) had mixed infections of the two species. The absence of C. parvum suggests that the age group of calves studied is not likely to be source of zoonotic infection to humans. |
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